High resolution in vivo imaging of microglia using a versatile non genetically-encoded marker.

We describe a technique utilizing Tomato lectin conjugated with green or red fluorescent dye for fast and reliable in vivo labeling of microglia. This protocol enables high resolution imaging of microglial cells in wild type/mutant mice of any age, and in mouse models of Alzheimer’s disease. The labeling does not disturb the functional properties of microglia or the surrounding neurons and is preserved in fixed tissue used for post-hoc immunostaining.

See more: http://www.ncbi.nlm.nih.gov/pubmed/22622946