Unique Functional Properties of Mature Adult-Born Neurons in the Mouse Olfactory Bulb

The rodent olfactory bulb (OB) is continuously supplied with adult-born cells maturing into GABAergic neurons. Using in vivo ratiometric Ca2+ imaging to readout ongoing and sensory-driven activity, we asked whether mature adult-born cells (mABCs) in the glomerular layer of the bulb become functionally identical to resident GABAergic (Res_GABA) neurons.

In awake head-restrained mice the two cell populations differed significantly in terms of ongoing spontaneous activity, with 24% of mABCs contributing to a strongly active cell cluster, absent among Res_GABA cells. Odor-evoked responses of mABCs were sparse, less reliable, and had smaller amplitudes compared with Res_GABA cells. The opposite was seen under anesthesia, with response reliability increasing and response size of mABCs becoming larger than that of Res_GABA cells. Furthermore, ongoing activity of mABCs showed increased sensitivity to ketamine/xylazine and was selectively blocked by the antagonist of serotonin receptors methysergide. These functional features of mABCs clearly distinguish them from other OB interneurons.

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